AMPLICONNOISE(1) AmpliconNoise Documentation AMPLICONNOISE(1)NAME
AmpliconNoise - remove noise from high throughput nucleotide sequence data
VERSION
This documentation refers to version 1.22
SYNOPSIS
See /usr/share/doc/ampliconnoise/Doc.pdf.gz for details of how to run.
DESCRIPTION
The following tools are included. Most of them have an MPI equivalent, for example SeqNoise has an equivalent SeqNoiseM which can be used
with mpirun.
FastaUnique - dereplicates fasta file
-in string input file name
Options:
FCluster
-in string distance input file name
-out string output file stub
Options:
-r resolution
-a average linkage
-w use weights
-i read identifiers
-s scale dist.
NDist - pairwise Needleman-Wunsch sequence distance matrix from a fasta file
-in string fata file name
Options:
-i output identifiers
Perseus - slays monsters
-sin string seq file name
Options:
-tin string reference sequence file
-a output alignments
-d use imbalance
-rin string lookup file name
PyroDist - pairwise distance matrix from flowgrams
-in string flow file name
-out stub out file stub
Options:
-ni no index in dat file
-rin string lookup file name
PyroNoise - clusters flowgrams without alignments
-din string flow file name
-out string cluster input file name
-lin string list file
Options:
-v verbose
-c double initial cut-off
-ni no index in dat file
-s double precision
-rin file lookup file name
SeqDist - pairwise distance matrix from a fasta file
-in string fasta file name
Options:
-i output identifiers
-rin string lookup file name
SeqNoise - clusters sequences
-in string sequence file name
-din string distance matrix file name
-out string cluster input file name
-lin string list file
Options:
-min mapping file
-v verbose
-c double initial cut-off
-s double precision
-rin string lookup file name
SplitClusterEven
-din string dat filename
-min string map filename
-tin string tree filename
-s split size
-m min size
AUTHOR
All software by Chris Quince (quince@civil.gla.ac.uk) This manpage by Tim Booth (tbooth@ceh.ac.uk)
LICENCE AND COPYRIGHT
Copyright (c) 2009 (quince@civil.gla.ac.uk). All rights reserved.
Released under the Lesser GPL.
Permission is granted for anyone to copy, use, or modify these programs and documents for purposes of research or education, provided this
copyright notice is retained, and note is made of any changes that have been made.
perl v5.12.4 2011-04-28 AMPLICONNOISE(1)
Check Out this Related Man Page
volume_injection(1) The Computational Morphometry Toolkit volume_injection(1)NAME
volume_injection - Volume injection
SYNOPSIS
volume_injection [options] refImage xform0 inImage0 [xform1 inImage1 ...]
DESCRIPTION
Reconstruction a high-resolution volume from multiple co-registered (low-resolution) images using forward volume injection
OPTIONS
Global Toolkit Options (these are shared by all CMTK tools)
--help
Write list of basic command line options to standard output.
--help-all
Write complete list of basic and advanced command line options to standard output.
--wiki
Write list of command line options to standard output in MediaWiki markup.
--man
Write man page source in 'nroff' markup to standard output.
--version
Write toolkit version to standard output.
--echo
Write the current command line to standard output.
--verbose-level <integer>
Set verbosity level.
--verbose, -v
Increment verbosity level by 1 (deprecated; supported for backward compatibility).
--threads <integer>
Set maximum number of parallel threads (for POSIX threads and OpenMP).
Input Options
--exclude-first-image, -x
Exclude first image from reconstruction as a separate registration target image)
--pass-weight <string>, -W <string>
Set contribution weight for a pass in the form 'pass:weight'
Reconstruction Grid Options
--recon-grid <string>
Define reconstruction grid as Nx,Ny,Nz:dX,dY,dZ[:Ox,Oy,Oz] (dims:pixel:offset)
--recon-grid-path <string>, -R <string>
Give path to grid that defines reconstructed image grid [including offset] [Default: NONE]
--crop <string>
Crop reference to pixel region x0,y0,z1:x1,y1,z1
Volume Injection Options
--isotropic-injection
Use isotropic volume injection [default: scaled with pass image pixel size per dimension]
--injection-kernel-sigma <double>, -S <double>
Gauss contribution [Default: 1]
--injection-kernel-radius <integer>, -r <integer>
VolumeInjectionRadius of affected pixel [Default: 0]
Output Options
--output <string>, -o <string>
Output image path [Default: volume_injection.nii ]
--write-images-as-float, -F
Write output images as floating point [default: same as input]
AUTHORS
Torsten Rohlfing, with contributions from Michael P. Hasak, Greg Jefferis, Calvin R. Maurer, and Daniel B. Russakoff
LICENSE
http://www.fsf.org/licensing/licenses/gpl.html
BUGS
Report bugs at http://nitrc.org/projects/cmtk/
ACKNOWLEDGMENTS
From April 2009 through September 2011, CMTK Development and Maintenance was supported by the National Institute of Biomedical Imaging and
Bioengineering under Grant No.R01 EB008381 (PI: Torsten Rohlfing).
CMTK 2.2.2 Jul 20 2012 volume_injection(1)