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BLASTCLUST(1)						     NCBI Tools User's Manual						     BLASTCLUST(1)

NAME
blastclust - BLAST score-based single-linkage clustering SYNOPSIS
blastclust [-] [-C] [-L X] [-S X] [-W N] [-a N] [-b F] [-c filename] [-d filename] [-e F] [-i filename] [-l filename] [-o filename] [-p F] [-r filename] [-s filename] [-v [filename]] DESCRIPTION
blastclust automatically and systematically clusters protein or DNA sequences based on pairwise matches found using the BLAST algorithm in case of proteins or Mega BLAST algorithm for DNA. In the latter case a single Mega BLAST search is performed for all the sequences combined against a database created from the same sequences. blastclust finds pairs of sequences that have statistically significant matches and clusters them using single-linkage clustering. OPTIONS
A summary of options is included below. - Print usage message -C Complete unfinished clustering -L X Length coverage threshold (default = 0.9) -S X Score coverage threshold (bit score / length if < 3.0, percentage of identities otherwise; default = 1.75) -W N Use words of size N (length of best perfect match; zero invokes default behavior: 3 for proteins, 32 for nucleotides) -a N Number of CPU's to use (default = 1) -b F Do not require coverage on both neighbours -c filename Read advanced options from configuration file filename -d filename Input as a database -e F Disable id parsing in database formatting -i filename FASTA input file (program will format the database and remove files in the end; default = stdin) -l filename Restrict reclustering to id list in filename -o filename Output file for list of clusters (default = stdout) -p F Input is nucleotides, not proteins. -r filename Restore neighbors for reclustering from filename -s filename Save all neighbours to filename -v [filename] Print verbose progress messages (to filename) AUTHOR
The National Center for Biotechnology Information. SEE ALSO
blast(1), formatdb(1), /usr/share/doc/blast2/blastclust.html, <http://www.ncbi.nlm.nih.gov/BLAST/> NCBI
2004-06-25 BLASTCLUST(1)

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ASN2FSA(1)						     NCBI Tools User's Manual							ASN2FSA(1)

NAME
asn2fsa - convert biological sequence data from ASN.1 to FASTA SYNOPSIS
asn2fsa [-] [-A acc] [-D] [-E] [-H] [-L filename] [-T] [-a type] [-b] [-c] [-d path] [-e N] [-f path] [-g] [-h filename] [-i filename] [-k] [-l] [-m] [-o filename] [-p path] [-q filename] [-r] [-s] [-u] [-v filename] [-x str] [-z] DESCRIPTION
asn2fsa converts biological sequence data from ASN.1 to FASTA. OPTIONS
A summary of options is included below. - Print usage message -A acc Accession to fetch -D Use Dash for Gap -E Extended Seq-ids -H HTML spans -L filename Log file -T Use Threads -a type Input ASN.1 type: a Automatic (default) z Any e Seq-entry b Bioseq s Bioseq-set m Seq-submit t batch processing (suitable for official releases; autodetects specific type) -b Bioseq-set is Binary -c Bioseq-set is Compressed -d path Path to ReadDB Database -e N Line length (70 by default; may range from 10 to 120) -f path Path to indexed FASTA data -g Expand delta gaps into Ns -h filename Far component cache output file name -i filename Single input file (standard input by default) -k Local fetching -l Lock components in advance -m Master style for near segmented sequences -o filename Nucleotide Output file name -p path Path to ASN.1 Files -q filename Quality score output file name -r Remote fetching from NCBI -s Far genomic contig for quality scores -u Recurse -v filename Protein output file name -x str File selection substring (.ent by default) [String] -z Print quality score gap as -1 AUTHOR
The National Center for Biotechnology Information. SEE ALSO
asn2all(1), asn2asn(1), asn2ff(1), asn2gb(1), asn2xml(1), asndhuff(1). NCBI
2011-09-02 ASN2FSA(1)
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