fastx_clipper(1) [debian man page]
FASTX_CLIPPER(1) User Commands FASTX_CLIPPER(1) NAME
fastx_clipper - FASTA/Q Clipper DESCRIPTION
usage: fastx_clipper [-h] [-a ADAPTER] [-D] [-l N] [-n] [-d N] [-c] [-C] [-o] [-v] [-z] [-i INFILE] [-o OUTFILE] Part of FASTX Toolkit 0.0.13.2 by A. Gordon (gordon@cshl.edu) [-h] = This helpful help screen. [-a ADAPTER] = ADAPTER string. default is CCTTAAGG (dummy adapter). [-l N] = discard sequences shorter than N nucleotides. default is 5. [-d N] = Keep the adapter and N bases after it. (using '-d 0' is the same as not using '-d' at all. which is the default). [-c] = Discard non-clipped sequences (i.e. - keep only sequences which contained the adapter). [-C] = Discard clipped sequences (i.e. - keep only sequences which did not contained the adapter). [-k] = Report Adapter-Only sequences. [-n] = keep sequences with unknown (N) nucleotides. default is to discard such sequences. [-v] = Verbose - report number of sequences. If [-o] is specified, report will be printed to STDOUT. If [-o] is not specified (and output goes to STDOUT), report will be printed to STDERR. [-z] = Compress output with GZIP. [-D] = DEBUG output. [-M N] = require minimum adapter alignment length of N. If less than N nucleotides aligned with the adapter - don't clip it. [-i INFILE] = FASTA/Q input file. default is STDIN. [-o OUTFILE] = FASTA/Q output file. default is STDOUT. SEE ALSO
The quality of this automatically generated manpage might be insufficient. It is suggested to visit http://hannonlab.cshl.edu/fastx_toolkit/commandline.html to get a better layout as well as an overview about connected FASTX tools. fastx_clipper 0.0.13.2 May 2012 FASTX_CLIPPER(1)
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FASTX_QUALITY_STATS(1) User Commands FASTX_QUALITY_STATS(1) NAME
fastx_quality_stats - FASTX Statistics DESCRIPTION
usage: fastx_quality_stats [-h] [-N] [-i INFILE] [-o OUTFILE] Part of FASTX Toolkit 0.0.13.2 by A. Gordon (gordon@cshl.edu) [-h] = This helpful help screen. [-i INFILE] = FASTQ input file. default is STDIN. [-o OUTFILE] = TEXT output file. default is STDOUT. [-N] = New output format (with more information per nucleotide/cycle). The *OLD* output TEXT file will have the following fields (one row per column): column = column number (1 to 36 for a 36-cycles read solexa file) count = number of bases found in this column. min = Lowest quality score value found in this column. max = Highest quality score value found in this column. sum = Sum of quality score values for this column. mean = Mean quality score value for this column. Q1 = 1st quartile quality score. med = Median quality score. Q3 = 3rd quartile quality score. IQR = Inter-Quartile range (Q3-Q1). lW = 'Left-Whisker' value (for boxplotting). rW = 'Right-Whisker' value (for boxplotting). A_Count = Count of 'A' nucleotides found in this column. C_Count = Count of 'C' nucleotides found in this column. G_Count = Count of 'G' nucleotides found in this column. T_Count = Count of 'T' nucleotides found in this column. N_Count = Count of 'N' nucleo- tides found in this column. max-count = max. number of bases (in all cycles) The *NEW* output format: cycle (previously called 'column') = cycle number max-count For each nucleotide in the cycle (ALL/A/C/G/T/N): count = number of bases found in this column. min = Lowest quality score value found in this column. max = Highest quality score value found in this column. sum = Sum of quality score values for this column. mean = Mean quality score value for this column. Q1 = 1st quartile quality score. med = Median quality score. Q3 = 3rd quartile quality score. IQR = Inter-Quartile range (Q3-Q1). lW = 'Left-Whisker' value (for boxplotting). rW = 'Right-Whisker' value (for boxplotting). SEE ALSO
The quality of this automatically generated manpage might be insufficient. It is suggested to visit http://hannonlab.cshl.edu/fastx_toolkit/commandline.html to get a better layout as well as an overview about connected FASTX tools. fastx_quality_stats 0.0.13.2 May 2012 FASTX_QUALITY_STATS(1)