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BP_LOAD_GFF(1p) 					User Contributed Perl Documentation					   BP_LOAD_GFF(1p)

NAME
bp_load_gff.pl - Load a Bio::DB::GFF database from GFF files. SYNOPSIS
% bp_load_gff.pl -d testdb -u user -p pw --dsn 'dbi:mysql:database=dmel_r5_1;host=myhost;port=myport' dna1.fa dna2.fa features1.gff features2.gff ... DESCRIPTION
This script loads a Bio::DB::GFF database with the features contained in a list of GFF files and/or FASTA sequence files. You must use the exact variant of GFF described in Bio::DB::GFF. Various command-line options allow you to control which database to load and whether to allow an existing database to be overwritten. This script uses the Bio::DB::GFF interface, and so works with all database adaptors currently supported by that module (MySQL, Oracle, PostgreSQL soon). However, it is slow. For faster loading, see the MySQL-specific bp_bulk_load_gff.pl and bp_fast_load_gff.pl scripts. NOTES If the filename is given as "-" then the input is taken from standard input. Compressed files (.gz, .Z, .bz2) are automatically uncompressed. FASTA format files are distinguished from GFF files by their filename extensions. Files ending in .fa, .fasta, .fast, .seq, .dna and their uppercase variants are treated as FASTA files. Everything else is treated as a GFF file. If you wish to load -fasta files from STDIN, then use the -f command-line swith with an argument of '-', as in gunzip my_data.fa.gz | bp_fast_load_gff.pl -d test -f - On the first load of a database, you will see a number of "unknown table" errors. This is normal. About maxfeature: the default value is 100,000,000 bases. If you have features that are close to or greater that 100Mb in length, then the value of maxfeature should be increased to 1,000,000,000, or another power of 10. COMMAND-LINE OPTIONS Command-line options can be abbreviated to single-letter options. e.g. -d instead of --database. --dsn <dsn> Data source (default dbi:mysql:test) --adaptor <adaptor> Schema adaptor (default dbi::mysqlopt) --user <user> Username for mysql authentication --pass <password> Password for mysql authentication --fasta <path> Fasta file or directory containing fasta files for the DNA --create Force creation and initialization of database --maxfeature Set the value of the maximum feature size (default 100 Mb; must be a power of 10) --group A list of one or more tag names (comma or space separated) to be used for grouping in the 9th column. --upgrade Upgrade existing database to current schema --gff3_munge Activate GFF3 name munging (see Bio::DB::GFF) --quiet No progress reports --summary Generate summary statistics for drawing coverage histograms. This can be run on a previously loaded database or during the load. SEE ALSO
Bio::DB::GFF, bulk_load_gff.pl, load_gff.pl AUTHOR
Lincoln Stein, lstein@cshl.org Copyright (c) 2002 Cold Spring Harbor Laboratory This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself. See DISCLAIMER.txt for disclaimers of warranty. perl v5.14.2 2012-03-02 BP_LOAD_GFF(1p)

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BP_SEQFEATURE_LOAD(1p)					User Contributed Perl Documentation				    BP_SEQFEATURE_LOAD(1p)

NAME
bp_seqfeature_load.pl - Load GFF into a SeqFeature database DESCRIPTION
Pass any number of GFF or fasta format files (or GFF with embedded fasta) to load the features and sequences into a SeqFeature database. The database (and adaptor) to use is specified on the command line. Use the --create flag to create a new SeqFeature database. SYNOPSIS
bp_seqfeature_load.pl [options] gff_or_fasta_file1 [gff_or_fasta_file2 [...]] Try 'bp_seqfeature_load.pl --help' or '--man' for more information. OPTIONS
-d, --dsn DBI data source (default dbi:mysql:test) -n, --namespace The table prefix to use (default undef) Allows several independent sequence feature databases to be stored in a single database -s, --seqfeature The type of SeqFeature to create... RTSC (default Bio::DB::SeqFeature) -a, --adaptor The storage adaptor (class) to use (default DBI::mysql) -v, --verbose Turn on verbose progress reporting (default true) Use --noverbose to switch this off. -f, --fast Activate fast loading. (default 0) Only available for some adaptors. -T, --temporary-directory Specify temporary directory for fast loading (default File::Spec->tmpdir()) -i, --ignore-seqregion If true, then ignore ##sequence-region directives in the GFF3 file (default, create a feature for each region) -c, --create Create the database and reinitialize it (default false) Note, this will erase previous database contents, if any. -u, --user User to connect to database as -p, --password Password to use to connect to database -z, --zip Compress database tables to save space (default false) -S, --subfeatures Turn on indexing of subfeatures (default true) Use --nosubfeatures to switch this off. --summary Generate summary statistics for coverage graphs (default false) This can be run on a previously loaded database or during the load. It will default to true if --create is used. --noalias-target Don't create an Alias attribute whose value is the target_id in a Target attribute (if the feature contains a Target attribute, the default is to create an Alias attribute whose value is the target_id in the Target attribute) Please see http://www.sequenceontology.org/gff3.shtml for information about the GFF3 format. BioPerl extends the format slightly by adding a ##index-subfeatures directive. Set this to a true value if you wish the database to be able to retrieve a feature's individual parts (such as the exons of a transcript) independently of the top level feature: ##index-subfeatures 1 It is also possible to control the indexing of subfeatures on a case-by-case basis by adding "index=1" or "index=0" to the feature's attribute list. This should only be used for subfeatures. Subfeature indexing is true by default. Set to false(0) to save lots of database space and speed performance. You may use --nosubfeatures to force this. perl v5.14.2 2012-03-02 BP_SEQFEATURE_LOAD(1p)
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