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bio::seq::largeprimaryseq(3pm) [debian man page]

Bio::Seq::LargePrimarySeq(3pm)				User Contributed Perl Documentation			    Bio::Seq::LargePrimarySeq(3pm)

NAME
Bio::Seq::LargePrimarySeq - PrimarySeq object that stores sequence as files in the tempdir (as found by File::Temp) or the default method in Bio::Root::Root SYNOPSIS
# normal primary seq usage DESCRIPTION
This object stores a sequence as a series of files in a temporary directory. The aim is to allow someone the ability to store very large sequences (eg, > 100MBases) in a file system without running out of memory (eg, on a 64 MB real memory machine!). Of course, to actually make use of this functionality, the programs which use this object must not call $primary_seq->seq otherwise the entire sequence will come out into memory and probably paste your machine. However, calls $primary_seq->subseq(10,100) will cause only 90 characters to be brought into real memory. FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHOR - Ewan Birney, Jason Stajich Email birney@ebi.ac.uk Email jason@bioperl.org APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ length Title : length Usage : Function: Example : Returns : Args : seq Title : seq Usage : Function: Example : Returns : Args : subseq Title : subseq Usage : Function: Example : Returns : Args : add_sequence_as_string Title : add_sequence_as_string Usage : $seq->add_sequence_as_string("CATGAT"); Function: Appends additional residues to an existing LargePrimarySeq object. This allows one to build up a large sequence without storing entire object in memory. Returns : Current length of sequence Args : string to append _filename Title : _filename Usage : $obj->_filename($newval) Function: Example : Returns : value of _filename Args : newvalue (optional) alphabet Title : alphabet Usage : $obj->alphabet($newval) Function: Example : Returns : value of alphabet Args : newvalue (optional) perl v5.14.2 2012-03-02 Bio::Seq::LargePrimarySeq(3pm)

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Bio::SeqIO::raw(3pm)					User Contributed Perl Documentation				      Bio::SeqIO::raw(3pm)

NAME
Bio::SeqIO::raw - raw sequence file input/output stream SYNOPSIS
Do not use this module directly. Use it via the Bio::SeqIO class. DESCRIPTION
This object can transform Bio::Seq objects to and from raw flat file databases. FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/ AUTHORS
Ewan Birney E<lt>birney@ebi.ac.ukE<gt> Lincoln Stein E<lt>lstein@cshl.orgE<gt> CONTRIBUTORS
Jason Stajich E<lt>jason@bioperl.org<gt> APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ next_seq Title : next_seq Usage : $seq = $stream->next_seq() Function: returns the next sequence in the stream Returns : Bio::Seq object Args : write_seq Title : write_seq Usage : $stream->write_seq($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Array of Bio::PrimarySeqI objects write_qual Title : write_qual Usage : $stream->write_qual($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Bio::Seq::Quality object variant Title : variant Usage : $format = $obj->variant(); Function: Get and set method for the sequence variant. For raw sequence, this indicates whether to treat the input as multiple sequences (the default) or as a single sequence. Current values accepted are: 'single' single sequence 'multiple' multiple sequences (default) Returns : string Args : new value, string perl v5.14.2 2012-03-02 Bio::SeqIO::raw(3pm)
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