SWISS::DEs(3pm) User Contributed Perl Documentation SWISS::DEs(3pm)
Name
SWISS::DEs.pm
Description
SWISS::DEs represents the DE lines of a UniProt Knowledgebase (Swiss-Prot + TrEMBL) entry as specified in the user manual
http://www.expasy.org/sprot/userman.html.
The DEs object basically holds lists of DE objects, each of them representing a protein name element. The "elements", "hasFragment",
"Includes" and "Contains" attributes/methods work as follows :
DE RecName: Full=CAD protein;
DE Short=CAD;
DE AltName: Full=Protein rudimentary;
DE Includes:
DE RecName: Full=Glutamine-dependent carbamoyl-phosphate synthase;
DE EC=6.3.5.5;
DE Includes:
DE RecName: Full=Aspartate carbamoyltransferase;
DE EC=2.1.3.2;
DE Flags: Fragment;
-= Entry::DEs =-
elements (for each DE object, see SWISS::DE.pm documentation) :
toText: "CAD protein", "CAD", "Protein rudimentary"
category: "RecName", "RecName", "AltName"
type: "Full", "Short" "Full"
hasFragment : "Fragment"
Includes : ListBase of DEs (child1, child2)
Contains : empty ListBase
-= child1 =-
elements (for each DE object) :
toText: "Glutamine-dependent carbamoyl-
phosphate synthase", "6.3.5.5"
category: "RecName", "RecName",
type: "Full", "EC"
hasFragment : undef
-= child2 =-
elements (for each DE object) :
toText: "Aspartate carbamoyltransferase", "2.1.3.2"
category: "RecName", "RecName",
type: "Full", "EC"
hasFragment : undef
Note: the old unstructured DE format can still be used, and will be parsed the same way into DE objects (but without setting their
attributes 'category' and 'type'.
DE CAD protein (Protein rudimentary) [Includes: Glutamine-dependent
DE carbamoyl-phosphate synthase (EC 6.3.5.5); Aspartate
DE carbamoyltransferase (EC 2.1.3.2)] (Fragment).
Inherits from
SWISS::ListBase.pm
Attributes
"text"
The (raw) text of the DE line (without the 'DE ' line type prefixes)
"list"
Array reference to the SWISS::DE objects containing the different names for the entry. The first element of the list is the recommended
name. Note: use "elements" method (inherited from ListBase) to get (and loop through) the array of DE objetcs.
"Includes"
"Contains"
Each of these is a SWISS::ListBase object whose list contains a SWISS::DEs object for each 'child' of the protein (i.e. peptide or
functional domain). See the UniProtKB user manual for an explanation. It is possible to have both Includes and Contains in a single
entry:
DE RecName: Full=Arginine biosynthesis bifunctional protein argJ;
DE Includes:
DE RecName: Full=Glutamate N-acetyltransferase;
DE EC=2.3.1.35;
DE AltName: Full=Ornithine acetyltransferase;
DE Short=OATase;
DE AltName: Full=Ornithine transacetylase;
DE Includes:
DE RecName: Full=Amino-acid acetyltransferase;
DE EC=2.3.1.1;
DE AltName: Full=N-acetylglutamate synthase;
DE Short=AGS;
DE RecName: Full=Arginine biosynthesis bifunctional protein argJ alpha chain;
DE Contains:
DE RecName: Full=Arginine biosynthesis bifunctional protein argJ beta chain;
"hasFragment"
Contains 'Fragment' or 'Fragments' (evaluates to true) if the DE lines contain the 'Fragment(s)' indication (in 'Flags:' line with the
new DE line format), otherwise evaluates to false. Compare to the more robust Entry::isFragment which also checks the FT lines for a
NON_CONS or NON_TER.
"isPrecursor"
Returns 1 if the flag 'Precursor' is present (undef if not). Note: only with new DE line format.
Methods
Standard methods
new
fromText
toText
Evidence Tags
With the new DE line format, each DE element can have distinct evidence tags, which are stored in the DE object themself (see SWISS::DE.pm
documentation). The evidence tags for the 'Flags' line are stored in the parent DEs object itself. With the old DE line format, since the
DE line did not have a fixed syntax in TrEMBL, it is impossible to reliably assign evidence tags separately to the different elements of
the DE lines. Therefore, the DE line can only be evidence tagged as a whole, and the following methods have their prototype defined in
SWISS::BaseClass instead of the direct parent of SWISS::DEs, SWISS::ListBase :
addEvidenceTag
deleteEvidenceTags
getEvidenceTags
getEvidenceTagsString
hasEvidenceTag
setEvidenceTags
example :
$evidenceTag = $entry->Stars->EV->addEvidence('P', 'DEfix', '-', 'v1.3');
# add global DE evtag if old DE line format, 'Flags' evtag if new format
$entry -> DEs -> addEvidenceTag($evidenceTag);
perl v5.10.1 2008-07-17 SWISS::DEs(3pm)