alistat(1) Biosquid Manual alistat(1)
NAME
alistat - show statistics for a multiple alignment file
SYNOPSIS
alistat [options] alignfile
DESCRIPTION
alistat reads a multiple sequence alignment from the file alignfile in any supported format (including SELEX, GCG MSF, and CLUSTAL), and
shows a number of simple statistics about it. These statistics include the name of the format, the number of sequences, the total number
of residues, the average and range of the sequence lengths, the alignment length (e.g. including gap characters).
Also shown are some percent identities. A percent pairwise alignment identity is defined as (idents / MIN(len1, len2)) where idents is the
number of exact identities and len1, len2 are the unaligned lengths of the two sequences. The "average percent identity", "most related
pair", and "most unrelated pair" of the alignment are the average, maximum, and minimum of all (N)(N-1)/2 pairs, respectively. The "most
distant seq" is calculated by finding the maximum pairwise identity (best relative) for all N sequences, then finding the minimum of these
N numbers (hence, the most outlying sequence).
OPTIONS
-a Show additional verbose information: a table with one line per sequence showing name, length, and its highest and lowest pairwise
identity. These lines are prefixed with a * character to enable easily grep'ing them out and sorting them. For example, alistat -a
foo.slx | grep * | sort -n +3 gives a ranked list of the most distant sequences in the alignment. Incompatible with the -f option.
-f Fast; use a sampling method to estimate the average %id. When this option is chosen, alistat doesn't show the other three pairwise
identity numbers. This option is useful for very large alignments, for which the full (N)(N-1) calculation of all pairs would be
prohibitive (e.g. Pfam's GP120 alignment, with over 10,000 sequences). Incompatible with the -a option.
-h Print brief help; includes version number and summary of all options, including expert options.
-q be quiet - suppress the verbose header (program name, release number and date, the parameters and options in effect).
-B (Babelfish). Autodetect and read a sequence file format other than the default (FASTA). Almost any common sequence file format is
recognized (including Genbank, EMBL, SWISS-PROT, PIR, and GCG unaligned sequence formats, and Stockholm, GCG MSF, and Clustal align-
ment formats). See the printed documentation for a complete list of supported formats.
EXPERT OPTIONS
--informat <s>
Specify that the sequence file is in format <s>, rather than the default FASTA format. Common examples include Genbank, EMBL, GCG,
PIR, Stockholm, Clustal, MSF, or PHYLIP; see the printed documentation for a complete list of accepted format names. This option
overrides the default format (FASTA) and the -B Babelfish autodetection option.
SEE ALSO
afetch(1), compalign(1), compstruct(1), revcomp(1), seqsplit(1), seqstat(1), sfetch(1), shuffle(1), sindex(1), sreformat(1), stranslate(1),
weight(1).
AUTHOR
Biosquid and its documentation are Copyright (C) 1992-2003 HHMI/Washington University School of Medicine Freely distributed under the GNU
General Public License (GPL) See COPYING in the source code distribution for more details, or contact me.
Sean Eddy
HHMI/Department of Genetics
Washington University School of Medicine
4444 Forest Park Blvd., Box 8510
St Louis, MO 63108 USA
Phone: 1-314-362-7666
FAX : 1-314-362-2157
Email: eddy@genetics.wustl.edu
Biosquid 1.9g January 2003 alistat(1)