glam2format(1) [debian man page]

GLAM2FORMAT(1)							   glam2 Manual 						    GLAM2FORMAT(1)

NAME

       glam2format - converts GLAM2 motifs to FASTA or MSF format

SYNOPSIS

       glam2format [options] my_format my_motif.glam2

       Formats: fasta, msf.

DESCRIPTION

       glam2format reads in a motif found by glam2, and writes it in a standard alignment format (FASTA-with-gaps or MSF). This enables the
       alignment to be passed to third-party software, including graphical visualization tools such as Kalignvu, Boxshade, and WebLogo. On the
       other hand, not all the motif information is preserved: in particular, the key positions are lost. Only the top motif in glam2 output is
       converted.

OPTIONS (DEFAULT SETTINGS)
       -o
	   Output file (stdout).

       -c
	   Make a compact alignment. By default, residues that are inserted between key positions are written as unaligned with each other. This
	   best reflects glam2's intention, but it can make the alignment large and full of gaps. With -c, inserted residues are written as
	   arbitrarily aligned with each other, just as they appear in the glam2 output.

       -f
	   Sequence file to make a "global" alignment by adding flanking sequences from the original FASTA-format sequence file. The flanking
	   sequences will be written as either unaligned with each other or arbitrarily aligned, depending on the -c option. The sequences should
	   have unique names and their order should be unchanged.

SEE ALSO

       boxshade(1), glam2(1), glam2mask(1), glam2-purge(1), glam2scan(1)

       The full Hypertext documentation of GLAM2 is available online at http://bioinformatics.org.au/glam2/ or on this computer in
       /usr/share/doc/glam2/.

REFERENCE

       If you use GLAM2, please cite: MC Frith, NFW Saunders, B Kobe, TL Bailey (2008) Discovering sequence motifs with arbitrary insertions and
       deletions, PLoS Computational Biology (in press).

AUTHORS

       Martin Frith
	   Author of GLAM2.

       Timothy Bailey
	   Author of GLAM2.

       Charles Plessy <plessy@debian.org>
	   Formatted this manpage in DocBook XML for the Debian distribution.

COPYRIGHT

       The source code and the documentation of GLAM2 are released in the public domain.

GLAM2 1056							    05/19/2008							    GLAM2FORMAT(1)

GLAM2-PURGE(1)							   glam2 Manual 						    GLAM2-PURGE(1)

NAME

       glam2-purge - Removes redundant sequences from a FASTA file

SYNOPSIS

       glam2-purge file score [options]

DESCRIPTION

       glam2-purge is a modified version of Andrew Neuwald's purge program that removes redundant sequences from a FASTA file. This is recommended
       in order to prevent highly similar sequences distorting the search for motifs. Purge works with either DNA or protein sequences and creates
       an output file such that no two sequences have a (gapless) local alignment score greater than a threshold specified by the user. The output
       file is named <file>.<score>. The alignment score is based on the BLOSUM62 matrix for proteins, and on a +5/-1 scoring scheme for DNA.
       Purge can also be used to mask tandem repeats. It uses the XNU program for this purpose.

OPTIONS

       -n
	   Sequences are DNA (default: protein).

       -b
	   Use blast heuristic method (default for protein).

       -e
	   Use an exhaustive method (default for DNA).

       -q
	   Keep first sequence in the set.

       -x
	   Use xnu to mask protein tandem repeats.

SEE ALSO

       glam2(1), glam2format(1), glam2mask(1), glam2scan(1), xnu(1)

       The full Hypertext documentation of GLAM2 is available online at http://bioinformatics.org.au/glam2/ or on this computer in
       /usr/share/doc/glam2/.

REFERENCES

       Purge was written by Andy Neuwald and is described in more detail in Neuwald et al., "Gibbs motif sampling: detection of bacterial outer
       membrane protein repeats", Protein Science, 4:1618-1632, 1995. Please cite it if you use Purge.

       If you use GLAM2, please cite: MC Frith, NFW Saunders, B Kobe, TL Bailey (2008) Discovering sequence motifs with arbitrary insertions and
       deletions, PLoS Computational Biology (in press).

AUTHORS

       Andrew Neuwald
	   Author of purge, renamed glam2-purge in Debian.

       Martin Frith
	   Modified purge to be ANSI standard C and improved the user interface.

       Timothy Bailey
	   Modified purge to be ANSI standard C and improved the user interface.

       Charles Plessy <plessy@debian.org>
	   Formatted this manpage in DocBook XML for the Debian distribution.

COPYRIGHT

       The source code and the documentation of Purge and GLAM2 are released in the public domain.

GLAM2 1056							    05/19/2008							    GLAM2-PURGE(1)